-
Figure 1. The yield of recombinant wild-type ATGL and mutants assessed by SDS-PAGE. A Protein expression of wild-type ATGL and mutants induced by IPTG. Wild-type ATGL, S47D, S87A and S87D (a), S47A and S87A (b), S430D and SMT3 (c), S430A and S430D (d) were expressed in bacteria cells. B Bacterial lysates of wild-type ATGL and mutants used for the TAG hydrolase assay. The content of ATGL and mutants was standardized by scanning the density of protein bands. See also the supplementary Fig. S1
-
Figure 2. The procedure of TAG hydrolase assay. Flow diagram of measuring the enzymatic activity of ATGL using adiposome platforms. This figure is reprinted by permission from Elsevier ref. (Ma et al. 2021)
-
Figure 3. The expected outcome of the TAG hydrolase assay. A Scheme of ATGL binding adiposomes that are radiolabeled by triolein [9,10-3H(N)] and catalysing TAG hydrolysis. The radiolabeled TAG was catalyzed to release radiolabeled oleic acid. B The enzymatic activities of wild-type ATGL and mutants measured using adiposome platforms. Data represent mean ± s.e.m., n = 3. ∗p < 0.05, ∗∗p < 0.01, two-tailed t-test. This figure is reprinted by permission from Elsevier ref. (Ma et al. 2021)
Figure
3 ,Table
9 个