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2008 Volume 17 Issue 6
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Zhang Hong-Yan, Lu Heng, Li Wei, Liang Ru-Qiang, Jin Kui-Juan, Zhou Yue-Liang, Ruan Kang-Cheng, Yang Guo-Zhen. 2008: Detection of microarray protein biomolecules by oblique-incidence reflectivity difference technique without labelling agents, Chinese Physics B, 17(6): 2288-2291.
Citation: Zhang Hong-Yan, Lu Heng, Li Wei, Liang Ru-Qiang, Jin Kui-Juan, Zhou Yue-Liang, Ruan Kang-Cheng, Yang Guo-Zhen. 2008: Detection of microarray protein biomolecules by oblique-incidence reflectivity difference technique without labelling agents, Chinese Physics B, 17(6): 2288-2291.
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Detection of microarray protein biomolecules by oblique-incidence reflectivity difference technique without labelling agents

  • Laboratory of Optical Physics, Institute of Physics and Center for Condensed Matter Physics, Chinese Academy of Sciences, Beifing,100190, China;Laboratory of Organic Optoelectronic Functional Materials and Molecular Engineering, Technical Institute of Phy

  • Laboratory of Optical Physics, Institute of Physics and Center for Condensed Matter Physics, Chinese Academy of Sciences, Beifing,100190, China

  • Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai,200031, China

  • Available Online: 30/06/2008
  • Fund Project: the Chinese Academy of Sciences for Key Topics in Innovation Engineering (Grant L3FX051W)
  • This paper reports that the detection to the protein in microarray format is carried out by oblique-incidence reflectivity difference (OI-RD) analysis without any labelling agents. The OI-RD intensities not only depend on the protein structure, but also vary with the protein concentration. The results indicate that this method should have potential application in detection of biochemical processes. The high throughout and in situ detection can be achieved by this method with further improving of the experimental system.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Detection of microarray protein biomolecules by oblique-incidence reflectivity difference technique without labelling agents

  • Available Online: 30/06/2008

Abstract: This paper reports that the detection to the protein in microarray format is carried out by oblique-incidence reflectivity difference (OI-RD) analysis without any labelling agents. The OI-RD intensities not only depend on the protein structure, but also vary with the protein concentration. The results indicate that this method should have potential application in detection of biochemical processes. The high throughout and in situ detection can be achieved by this method with further improving of the experimental system.

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